CLOSE LINKAGE OF THE DOMINANT CATARACT MUTATIONS (CAT-2) WITH IDH-1 AND CRYGE ON MOUSE CHROMOSOME-1

The murine dominant gene Cat-2 was located on chromosome 1 between the loci of fuzzy and leaden. Subsequent linkage analysis revealed one re combinant between Cat-2(t) and isocitrate dehydrogenase-1, and one bet ween Cat-2(t) and gamma E-crystallin among 338 offspring in three-poin t backcrosses. The resulting genetic distance between the loci is 0.3 +/- 0.3 cM. The very close linkage between the Cat-2 and the gamma-cry stallin gene cluster together with the finding of reduced gamma-crysta llin transcripts in mutant lenses suggest strongly that the gamma-crys tallin genes may be candidate genes for the Cat-2 mutations. (C) 1994 Academic Press, Inc.