GENOMIC CLONING OF THE MOUSE LDL RECEPTOR-RELATED PROTEIN ALPHA(2)-MACROGLOBULIN RECEPTOR GENE

The LDL receptor-related protein (LRP) or alpha(2)-macroglobulin recep tor (A2mr) is encoded by a 15-kb mRNA in mouse and human. Probes encom passing different regions of the mouse cDNA were used to isolate clone s from a cosmid library of mouse strain 129. Four overlapping cosmids were used for restriction mapping and Southern blot analysis. This map and hybridization data obtained with oligonucleotide probes from the 5' and 3' ends of the Lrp cDNA demonstrated that the mouse gene is app roximately 85 kb in size. The Lrp promoter region was sequenced and re veals strong evolutionary conservation of putative regulatory elements between mouse and human. The present study will facilitate detailed e lucidation of the function of LRP in vivo. (C) 1994 Academic Press, In c.