CHROMOSOME MAPPING OF THE HUMAN ARRESTIN (SAG), BETA-ARRESTIN-2 (ARRB2), AND BETA-ADRENERGIC-RECEPTOR KINASE-2 (ADRBK2) GENES

Two types of proteins play a major role in determining homologous dese nsitization of G-coupled receptors: beta-adrenergic receptor kinase (b eta ARK), which phosphorylates the agonist-occupied receptor and its f unctional cofactor, beta-arrestin. Both beta ARK and beta-arrestin are members of multigene families. The family of G-protein-coupled recept or kinases includes rhodopsin kinase, beta ARK1, beta ARK2, IT11-A (GR K4), GRK5, and GRK6. The arrestin/beta-arrestin gene family includes a rrestin (also known as S-antigen), beta-arrestin 1, and beta-arrestin 2. Here we report the chromosome mapping of the human genes for arrest in (SAG), beta-arrestin 2 (ARRB2), and beta ARK2 (ADRBK2) by fluoresce nce in situ hybridization (FISH). FISH results confirmed the assignmen t of the gene coding for arrestin (SAG) to chromosome 2 and allowed us to refine its localization to band q37. The gene coding for beta-arre stin 2 (ARRB2) was mapped to chromosome 17p13 and that coding for beta ARK2 (ADRBK2) to chromosome 22q11. (C) 1994 Academic Press, Inc.