ADSORPTION OF BOVINE SERUM-ALBUMIN ONTO ACRYLAMIDE-MALEIC ACID HYDROGELS

In this study, acrylamide-maleic acid hydrogels containing different a mounts of maleic acid have been prepared by irradiating with gamma rad iation. They have been used in experiments on bovine serum albumin (BS A) adsorption. Acrylamide-maleic acid hydrogel containing 60 mg of mal eic acid and irradiated at 5.20 kGy has been used for swelling and dif fusion studies in water, solutions of NaCl and BSA. For this hydrogel, maximum and minimum swellings have been observed with water (1680%) a nd the solution of BSA (1085%), respectively. Diffusions of water, NaC l and BSA within hydrogels have been found as non-Fickian types of dif fusion. In the experiments of BSA adsorption, Langmuir type adsorption has been found. The hydrogel prepared with 60 mg maleic acid and havi ng been irradiated at 2.00 kGy has been found to be the best adsorptio n system for BSA. The adsorption capacity of acrylamide hydrogel has b een increased by the addition of maleic acid almost 1.5-3-fold.