Uncommon and controversial structures in cattle oocytes were studied i
n dominant and subordinate follicles of unstimulated Bos taurus and ze
bu (Bos indicus), and in FSH-stimulated B. taurus cattle, before or af
ter administration of cloprostenol. Growing oocytes were very rare in
follicles more than or equal to 5 mm in diameter. For the first time,
special vesicles that may be involved in cortical granule synthesis we
re observed. Classical rough endoplasmic reticulum was very rare in un
stimulated oocytes, but was seen at superovulation. Vacuolation of the
nucleolus was a common feature in dominant oocytes that were collecte
d after cloprostenol injection, except in superovulated cattle. Annula
te lamellae were very rare or absent in oocytes of B. taurus, while th
ey were more frequent in zebu oocytes.