Mg. Lambrechts et al., MULTIPLE POSITIVE AND NEGATIVE CIS-ACTING ELEMENTS OF THE STA2 GENE REGULATE GLUCOAMYLASE SYNTHESIS IN SACCHAROMYCES-CEREVISIAE, Gene, 146(2), 1994, pp. 137-144
Expression of the glucoamylase-encoding gene (STA2) in Saccharomyces c
erevisiae was previously shown to be regulated transcriptionally by bo
th positive and negative factors. The objective of this work was to id
entify the cis-acting elements responsible for STA2 transcriptional ac
tivation as well as the transcriptional repressor effects of STA10 and
MATa/MAT alpha. We identified two upstream activation regions (UAS).
Three repressor regions responsive to STA10-mediated repression were i
dentified, as well as two regions for down-regulation of STA2 expressi
on. MATa/MAT alpha repression appears to effect STA2 expression either
downstream from the translational start site or, indirectly, since no
functional a1/alpha 2responsive sequence was identified in the promot
er region.