CAPILLARY ELECTROPHORESIS ELECTROSPRAY MASS-SPECTROMETRY USING A HIGH-PERFORMANCE MAGNETIC-SECTOR MASS-SPECTROMETER

Capillary electrophoresis/electrospray mass spectrometry using a high- performance magnetic sector mass spectrometer has been performed with both bare fused silica and 3-(aminopropyl)trimethoxysilane-derivatized silica columns for simple peptide mixtures and for a snake venom. The latter is essentially a naturally occurring complex mixture of small proteins. Adaptation of the existing electrospray system to accommodat e capillary electrophoresis methodologies was extremely simple. Full-s can CE/MS on a sector mass spectrometer combined sensitivity with high resolution for discrimination between species of similar mass to char ge ratios.