The analysis of aqueous H2O2 at concentrations as low as 1 mu M is con
veniently done by the I-3(-) method, which is based on the spectrophot
ometric determination of I-3(-) formed when H2O2 is added to a concent
rated solution of I-. At 351 nm, epsilon(max) (I-3(-)) was measured to
be 26 450 M(-1) cm(-1). By contrast, an apparent value of 25 800 M(-1
) cm(-1) was determined from a calibration of the I-3(-) method agains
t titration by permanganate. The difference could only be partially ac
counted for by the equilibrium between I-3(-), I-2, and I-. A further
correction of similar to 1% was required and was traced to a side reac
tion between H2O2 and the buffer normally used in the I-3(-) method. A
simple spectrophotometric procedure was developed which improves the
sensitivity of the permanganate titration to 0.3 mu M H2O2. Measuremen
ts of H2O2 using the oxidation of ferrous ions (Fricke solution) and p
ermanganate titration differed by less than 1%.