T. Hayashi et al., EFFECTS OF THE DEGREE OF POLYMERIZATION ON THE BINDING OF XYLOGLUCANSTO CELLULOSE, Plant and Cell Physiology, 35(6), 1994, pp. 893-899
Xyloglucan oligosaccharides were isolated with various degrees of poly
merization (DP) and reduced with tritiated sodium borohydride. The H-3
-oligosaccharides were tested for their ability to bind to amorphous a
nd microcrystalline celluloses and to cellulose filter paper. The time
course of binding indicated that the radiolabeled oligosaccharides co
ntinued to be bound for at least 1 h after heating at 120 degrees C. T
he binding probably required the organization of the oligosaccharides
and celluloses by gradual annealing after heating. Although neither pe
ntasaccharide (glucose: xylose, 3:2), heptasaccharide (glucose:xylose,
4:3) and nonasaccharide (glucose:xylose: galactose:fucose, 4:3:1 : 1)
failed to bind to the celluloses, binding occurred with oligosacchari
des with DP equivalent to more than four consecutive 1,4-beta-glucosyl
residues. The extent of binding to the celluloses increased gradually
from octasaccharide (glucose:xylose, 5:3) to hendecosanosaccharide (g
lucose/xylose, 12:9), with the increase in the DP of 1,4-beta-glucosyl
residues. The binding of reduced cello-dextrins to cellulose required
at least 4 consecutive 1,4-beta-glucosyl residues. The extent of bind
ing of cellopentitol or cellohexitol to cellulose was similar to that
of hendecosanosaccharide, showing lower binding for xyloglucan oligosa
ccharides in spite of longer chains of 1,4-beta-glucosyl residues. The
se findings suggest that the mode of binding to cellulose of xylogluca
n oligosaccharides is different from that of cello-oligosaccharides.