THE RHESUS MACAQUE BETA-1-ADRENERGIC RECEPTOR GENE - STRUCTURE OF THEGENE AND COMPARISON OF THE FLANKING SEQUENCES WITH THE RAT BETA-1-ADRENERGIC RECEPTOR GENE

We have cloned the gene for the rhesus macaque beta(1)-adrenergic rece ptor. In addition to the protein coding block, we have sequenced its 5 ' (1424 bp) and 3' (1534 bp) flanking regions and aligned them with co mparable sections of the rat beta(1)-adrenergic receptor gene. The rhe sus macaque gene contains a 1440 bp open reading frame which codes for a deduced protein of 480 amino acids that is 95% and 89% similar to t he human and rat beta(1)-adrenergic receptors, respectively. The rhesu s macaque beta(1)-adrenergic receptor contains conserved sites for pot ential N-linked glycosylation and cAMP-dependent protein kinase phosph orylation identified within the human and rat receptors, but differs i n the structure and length of the third cytoplasmic loop. The 400 base s of 5' flanking sequence proximal to the protein coding block are hig hly conserved (84% similarity) between the rat and rhesus macaque gene s. The entire 3' flanking sequence, which extends beyond two potential polyadenylation sites at 1050 and 1337 bp relative to the translation termination codon, is also highly conserved between the two species. Comparison of the flanking sequences of the two species reveals conser ved regulatory sequences which may be important for beta(1)-adrenergic receptor expression and transcriptional modulation.