SEQUENCE PATTERNS AND HYBRIDIZATION ANALYSIS OF CLONES GENERATED BY ALU-PCR

A series of clones from an Alo-PCR library were analysed in more detai l. Characterization by Southern blot hybridization and sequencing disp layed several features common to all probes generated by this approach : Short length of the PCR-products as well as the presence of homologo us regions on both ends resulted in a limited feasability for filter h ybridization and a low probability of restriction length polymorphisms . In addition, a series of different short repeats at the 3'-ends of A lu-repeats and present in the generated probes offers a rich source of potential variable sites accessible by PCR.