NUCLEOTIDE-SEQUENCE OF THE GENE FOR PEPTOSTREPTOCOCCAL PROTEIN-L

Authors
MURPHY JP TROWERN AR DUGGLEBY CJ
Citation
Jp. Murphy et al., NUCLEOTIDE-SEQUENCE OF THE GENE FOR PEPTOSTREPTOCOCCAL PROTEIN-L, DNA sequence, 4(4), 1994, pp. 259-265
Citations number
34
Categorie Soggetti
Biothechnology & Applied Migrobiology","Genetics & Heredity
Journal title
DNA sequence → ACNP
ISSN journal
10425179
Volume
4
Issue
4
Year of publication
1994
Pages
259 - 265
Database
ISI
SICI code
1042-5179(1994)4:4<259:NOTGFP>2.0.ZU;2-2
Abstract
A gene bank of Peptostreptococcus magnus DNA was established using an E. coli host-vector system. Western blot analysis identified a clone e xpressing protein L which bound to the light chain of human immunoglob ulins. DNA sequence determination and analysis revealed an open readin g frame of 992 amino acids, giving a theoretical secreted protein of 1 06 kD with a pl of 4.67.