The molecular characterization of 28 clinical Actinomyces pyogenes str
ains was attempted. SDS-PAGE protein profiles did not allow to disting
uish isolates. Restriction endonuclease analysis of total DNA gave the
finest differentiation between strains but the profiles were difficul
t to read. Ribotypes after DNA digestion by Bst EII or Sma I have a hi
gh discriminatory power and are more helpful epidemiological markers.
No relationship could be demonstrated between molecular types and clin
ical sources.