MOLECULAR TYPING OF ACTINOMYCES-PYOGENES ISOLATES

Citation
V. Guerinfaublee et al., MOLECULAR TYPING OF ACTINOMYCES-PYOGENES ISOLATES, Zentralblatt fur Bakteriologie, 281(2), 1994, pp. 174-182
Citations number
22
Categorie Soggetti
Microbiology,Virology
ISSN journal
09348840
Volume
281
Issue
2
Year of publication
1994
Pages
174 - 182
Database
ISI
SICI code
0934-8840(1994)281:2<174:MTOAI>2.0.ZU;2-I
Abstract
The molecular characterization of 28 clinical Actinomyces pyogenes str ains was attempted. SDS-PAGE protein profiles did not allow to disting uish isolates. Restriction endonuclease analysis of total DNA gave the finest differentiation between strains but the profiles were difficul t to read. Ribotypes after DNA digestion by Bst EII or Sma I have a hi gh discriminatory power and are more helpful epidemiological markers. No relationship could be demonstrated between molecular types and clin ical sources.