INVOLVEMENT OF HUMAN-LEUKOCYTE ANTIGEN CLASS-I MOLECULES IN HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION OF CD4-POSITIVE CELLS

We have studied the putative roles of human immunodeficiency virus (HI V) associated and cell surface-expressed major histocompatibility comp lex class I (MHC-I) molecules in the course of the HIV life cycle by t he combined use of MHC-I molecule-positive and MHC-I molecule-negative virus particles and MHC-I molecule-positive and MHC-I molecule-negati ve CD4(+) human cells. We found (i) that several anti-MHC-I monoclonal antibodies neutralize cell infection by direct interaction with HIV-a ssociated MHC-I antigens, (ii) that these HIV-associated MHC-I antigen s are however dispensable for cell infection, and (iii) that the cell surface-expressed MHC-I molecules are unnecessary for productive infec tion of CD4(+) human cells. These results clarify further the function s of MHC-I molecules during the HIV life cycle.