RECOMBINANT RABBIT HEMORRHAGIC-DISEASE VIRUS CAPSID PROTEIN EXPRESSEDIN BACULOVIRUS SELF-ASSEMBLES INTO VIRUS-LIKE PARTICLES AND INDUCES PROTECTION

Authors
LAURENT S VAUTHEROT JF MADELAINE MF LEGALL G RASSCHAERT D
Citation
S. Laurent et al., RECOMBINANT RABBIT HEMORRHAGIC-DISEASE VIRUS CAPSID PROTEIN EXPRESSEDIN BACULOVIRUS SELF-ASSEMBLES INTO VIRUS-LIKE PARTICLES AND INDUCES PROTECTION, Journal of virology, 68(10), 1994, pp. 6794-6798
Citations number
34
Categorie Soggetti
Virology
Journal title
Journal of virology → ACNP
ISSN journal
0022538X
Volume
68
Issue
10
Year of publication
1994
Pages
6794 - 6798
Database
ISI
SICI code
0022-538X(1994)68:10<6794:RRHVCP>2.0.ZU;2-O
Abstract
VP60, the unique component of rabbit hemorrhagic disease virus capsid, was expressed in the baculovirus system. The recombinant VP60, releas ed in the supernatant of infected insect cells, assembled without the need of any other viral component to form viruslike particles (VLPs), structurally and immunologically indistinguishable from the rabbit hem orrhagic disease virion. Intramuscular vaccination of rabbits with the VLPs conferred complete protection in 15 days; this protection was fo und to be effective from the fifth day after VLP injection and was acc ompanied by a strong humoral response.