Na. Krucher et Mh. Roberts, IDENTIFICATION OF CDK-LIKE AND CYCLIN-LIKE PROTEINS IN THE EYE OF BULLA-GOULDIANA, Journal of neurobiology, 25(10), 1994, pp. 1200-1206
The ocular circadian rhythm in the eye of Bulla gouldiana is generated
by a rhythm in membrane potential of retinal neurons that is driven b
y alterations in potassium conductance. Since potassium conductance ma
y be modulated by the phosphorylation of potassium channels, the circa
dian rhythm may reflect rhythmic changes in protein kinase activity. F
urthermore, the circadian rhythm recorded from the Bulla eye can be ph
ase shifted by agents that affect protein synthesis and protein phosph
orylation on tyrosine residues. Interestingly, the eukaryotic cell div
ision cycle is generated by similar processes. Rhythmic cell division
is regulated by periodic synthesis and degradation of a protein, cycli
n, and periodic tyrosine phosphorylation of a cyclin-dependent kinase
(cdk), p34(cdc2). The interaction between these two proteins results i
n rhythmic kinase activity of p34(cdc2). Both cyclin and p34(cdc2) are
part of two diverse gene families, some of whose members have been lo
calized to postmitotic cell types with no function yet determined. In
the current work, we identify proteins similar to the cdks and cyclin
in the eye of Bulla. Neither of these ocular proteins are found in mit
otic cells in Bulla, and the cdk-like protein (p40) is specific to the
eye. Furthermore, the concentration of the cyclin-like protein (p66)
is affected by treatments that phase shift the circadian rhythm. The i
dentification of cdk and cyclin-like proteins in the Bulla eye is cons
istent with the hypothesis that the biochemical mechanism responsible
for generating the ocular circadian rhythm in Bulla is related to the
biochemical mechanism that regulates the eukaryotic cell division cycl
e. (C) 1994 John Wiley and Sons, Inc.