The objective of this study was to evaluate whether the suspension of
sperm naturally bound or artificially coated with antisperm antibody (
ASA) in test yolk buffer (TYB) will reduce the percentage of sperm pos
itive for ASA. Attempts were made to repeat a study performed by Lam e
t al., in which they claimed a significant decrease in percentage of s
perm positive for both IgA and IgG. Furthermore, the study attempted t
o determine if this methodology would similarly reduce the percentage
of sperm naturally bound with ASA. There was no reduction in postincub
ation levels in percentage of sperm having attachment of IgA or IgG in
either the sperm specimens naturally bound with ASA or donor sperm ar
tificially coated with antibodies. Incubation in TYB does not appear t
o be an effective method to reduce the percentage of sperm bound with
ASA. Possibly, it could reduce the amount of ASA bound to each sperm,
but this would have to be proven by other studies.