Ja. Kumari et T. Panda, INTERGENERIC HYBRIDIZATION OF TRICHODERMA-REESEI QM9414 AND SACCHAROMYCES-CEREVISIAE NCIM-3288 BY PROTOPLAST FUSION, Enzyme and microbial technology, 16(10), 1994, pp. 870-882
An attempt to produce ethanol from a cellulosic substrate by a single-
stage process using intergeneric hybrids obtained from Trichoderma ree
sei QM 9414 and Saccharomyces cerevisiae NCIM 3288 fusants is describe
d. Two hundred one fused strains were isolated using two different com
binations of hybridization, followed by polyethylene glycol treatment
of protoplasts derived fram parent and mutant strains. Some fusants we
re either diploids or heterokaryons, as verified by a protoplast fused
system obtained by auxotrophic mutants derived from T. reesei QM 9414
and S. cerevisiae NCIM 3288. Of the 201 fusants, 170 were found to be
relatively stable. Two fusants (M14 and M62) showed the highest synth
esis of ethanol from filter paper cellulose, producing 0.0149 and 0.01
91 gl(-1), respectively.