Jc. Garberi et al., USE OF A MONOCLONAL-ANTIBODY AND A DNA-PROBE FOR DIAGNOSING ACUTE TOXOPLASMOSIS IN AMBIGUOUS CASES, Journal of Clinical Pathology, 47(9), 1994, pp. 853-854
An unusual case of cerebral toxoplasmosis in an HIV negative 11 year o
ld child is reported. Central nervous system disease was assessed immu
nohistochemically in a brain biopsy specimen with TgP8-a specific mono
clonal antibody against Toxo-plasma gondii antigens-thus confirming Ig
G and IgM serology, technetium scan findings, and clinical data. In ad
dition, an active parasitaemia was confirmed by DNA in situ hybridisat
ion assay in white cells using an ABGTg4 probe. The child recovered af
ter specific T gondii treatment. Follow up six months later showed tha
t he was immunodeficient.