IN-SITU HYBRIDIZATION FOR THE IDENTIFICATION OF HELICOBACTER-PYLORI IN PARAFFIN WAX EMBEDDED TISSUE

A method for identifying Helicobacter pylori using a non-isotopic in s itu hybridisation technique is described. A probe generated by polymer ase chain reaction (PCR) with primers directed against parts of the He licobacter pylori 16SrRNA sequence was used. Paraffin wax embedded gas tric biopsy specimens from patients with and without gastritis were hy bridised with the probe, and the method was shown to be sensitive and specific for H pylori.