MONOCLONAL-ANTIBODIES AGAINST GOLDFISH (CARASSIUS-AURATUS) IMMUNOGLOBULIN - APPLICATION TO THE QUANTIFICATION OF IMMUNOGLOBULIN AND ANTIBODY-SECRETING CELLS BY ELISPOT AND SERIC IMMUNOGLOBULIN AND ANTIBODY-LEVELS BY ELISA IN CARP (CYPRINUS-CARPIO)

Authors
SIWICKI AK VERGNET C CHARLEMAGNE J DUNIER M
Citation
Ak. Siwicki et al., MONOCLONAL-ANTIBODIES AGAINST GOLDFISH (CARASSIUS-AURATUS) IMMUNOGLOBULIN - APPLICATION TO THE QUANTIFICATION OF IMMUNOGLOBULIN AND ANTIBODY-SECRETING CELLS BY ELISPOT AND SERIC IMMUNOGLOBULIN AND ANTIBODY-LEVELS BY ELISA IN CARP (CYPRINUS-CARPIO), Veterinary research, 25(5), 1994, pp. 458-467
Citations number
22
Categorie Soggetti
Veterinary Sciences
Journal title
Veterinary research → ACNP
ISSN journal
09284249
Volume
25
Issue
5
Year of publication
1994
Pages
458 - 467
Database
ISI
SICI code
0928-4249(1994)25:5<458:MAG(I>2.0.ZU;2-0
Abstract
Monoclonal antibodies (mAbs) raised against heavy and light chains of goldfish immunoglobulin (Ig) were characterized by a Western blot tech nique. A complete cross-reactivity was observed between carp and goldf ish Ig. These mAbs were used for the quantification of carp Ig and ant i-Yersinia ruckeri antibodies by ELISA. An ELISPOT assay was also deve loped in carp to quantify Ig-secreting cells (ISC) and antibody-secret ing cells (ASC). The number of ASC was maximum on day 18 post-vaccinat ion and decreased to the basal level on day 28. The antibody levels in sera were maximum on day 18 and slowly decreased until day 28.