BIOELECTROCATALYTICAL DETERMINATION OF SOME HEAVY-METALS AND THEIR INFLUENCE ON THE CATALYTIC ACTIVITY OF THE IMMOBILIZED ACETYLCHOLINESTERASE

On the basis of the process of the acetylthiocholine iodide hydrolysis , catalysed by the enzyme acetylcholinesterase and the obtaining of an electroactive product, an amperometric sensor is created. An electroc hemically modified graphite electrode is used, with acetylcholinestera se covalently linked directly on its surface. It is demonstrated that it can be applied for the quantitative determination of some heavy met al ions - Fe3+, Cd2+, Cu2+ and Mn2+ and for establishing their influen ce on the acetylcholinesterase activity.