S. Kintzios et G. Fischbeck, ANTHER CULTURE RESPONSE OF HORDEUM SPONTANEUM-DERIVED WINTER BARLEY LINES, Plant cell, tissue and organ culture, 37(2), 1994, pp. 165-170
The anther culture response of 18 Hordeum spontaneum derived winter ba
rley lines, as well as that of the F-2 and/or BC1 progeny of the cross
between nine of these lines and the winter barley cultivar Triton was
assessed. While the majority of lines showed a poor regeneration capa
city (4%), two lines, RS 170-A13 x Sonja and RS 120-27 x Sonja demonst
rated an outstanding response, producing 26 and 8 green regenerants pe
r 100 anthers plated, respectively. Five further lines, RS 70-30 x Dur
a, RS L74-11 x Dura, RS 122-19 x Sonja. RS 142-29 x Dura and RS 170-A5
1 x Sonja were identified as highly responsive in the F-2 generation.
An average production of 4 green regenerants per 100 anthers plated wa
s observed both in the parental and F-2 generation.