CHARACTERIZATION OF A MUTATED P53 EXHIBITING A CELL CYCLE-RELATED NUCLEAR TRANSLOCATION

We studied the p53 expression in the F4NW0 cell line. We found that th is mutated p53 has a deletion of eight amino acids, between the conser ved domains IV and V. Only one of the two alleles is expressed. It con tains a single base mutation, which seems to promote the use of a cryp tic splicing acceptor site, resulting in the observed deletion. The pr otein shows a cell cycle-dependent nuclear translocation: in G(1), it is exclusively cytoplasmic, and during the G(1)/S transition, it trans locates into the nucleus. Nevertheless, different antibodies assayed r evealed different patterns of localization.