Tc. Hsieh et al., MODULATION OF PROLIFERATION AND CELLULAR-CHANGES OF HUMAN PROSTATIC-CANCER CELLS BY ACTIVATED LYMPHOCYTES, International journal of oncology, 5(4), 1994, pp. 979-984
The effect of immune cells on the growth and development of human pros
tatic cancer cells was investigated. Cell proliferation of androgen-in
dependent human prostatic cancer cells JCA-1 was reduced 30-40% with t
he supplementation of a growth modulating activity present in lymphocy
te conditioned medium. The slower growing cells had a longer doubling
time revealing a blocking for G(1) cells entering into S phase. The cl
onogenicity of the cells was also reduced. Displayed in parallel was a
ltered phenotypes including the development of dendrite-like processes
with less intercellular contact. Immunocytochemical staining showed s
ignificantly increased: expression of actin, vimentin and cytokeratin
8, suggesting a differentiated cell type. Activated lymphocytes were s
hown to require RNA synthesis for providing the modulating activity wh
ich was estimated to be 18,500-27,500 Da. The role of immune cells in
growth regulation of prostatic cells is discussed.