D. Ramakrishnan et al., INOCULATION AND TISSUE DISTRIBUTION IN PILOT-SCALE PLANT-ROOT CULTUREBIOREACTORS, Biotechnology techniques, 8(9), 1994, pp. 639-644
Inoculation of large-scale plant root culture reactors can be carried
out by briefly homogenizing bulk root tissue, followed by aseptic tran
sfer as a slurry to the reactor. Uniform root distribution can be achi
eved in bioreactors by entrapment of the growing root inoculum onto pr
ocess packing elements (e.g. distillation packings), randomly distribu
ted within the reactor, in a bubble column operation. These two techni
ques have been successfully used to inoculate a 14 L pilot-scale react
or which is subsequently operated as a trickle bed reactor.