COPPER AFFINITY PRECIPITATION AS AN INITIAL STEP IN PROTEIN-PURIFICATION

Authors
AGARWAL R GUPTA MN
Citation
R. Agarwal et Mn. Gupta, COPPER AFFINITY PRECIPITATION AS AN INITIAL STEP IN PROTEIN-PURIFICATION, Biotechnology techniques, 8(9), 1994, pp. 655-658
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
9
Year of publication
1994
Pages
655 - 658
Database
ISI
SICI code
0951-208X(1994)8:9<655:CAPAAI>2.0.ZU;2-J
Abstract
The affinity of copper ions for histidine residues can be utilized to carry out fractional precipitation of proteins. Using this approach it is possible to purify concanavalin A by a simple two step procedure. The fold purification obtained was 4.5 fold.