A NOVEL DEVICE FOR THE ASSESSMENT OF NORMAL STRESSES TIME VARIATIONS EFFECTS ON SUSPENDED CELL - DESIGN AND PERFORMANCES

Authors
BAVOUZET JM LAFFORGUEDELORME C FONADE C GOMA G
Citation
Jm. Bavouzet et al., A NOVEL DEVICE FOR THE ASSESSMENT OF NORMAL STRESSES TIME VARIATIONS EFFECTS ON SUSPENDED CELL - DESIGN AND PERFORMANCES, Biotechnology techniques, 8(9), 1994, pp. 663-670
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
9
Year of publication
1994
Pages
663 - 670
Database
ISI
SICI code
0951-208X(1994)8:9<663:ANDFTA>2.0.ZU;2-K
Abstract
A generator of strictly normal stress time variations was designed in order to study the membrane resistance to mechanical stress of various cell species. This system provides pressure drops within very short t ime periods : 22 MPa in 1.9 msec in liquid systems and 6 MPa in 10 mse c in the presence of a gas. It also offers the possibility to study th e effect of successive ''compression / decompression'' cycles on cell integrity and metabolism. A single decompression provoked no structura l damage to the cells.