Rj. Lara et Dn. Thomas, XAD-FRACTIONATION OF NEW DISSOLVED ORGANIC-MATTER - IS THE HYDROPHOBIC FRACTION SERIOUSLY UNDERESTIMATED, Marine chemistry, 47(1), 1994, pp. 93-96
Freshly produced radiolabelled dissolved organic carbon (PDOC) from a
marine diatom culture was fractionated on XAD-2 by NaOH/methanol eluti
on. A large portion (32%) of the hydrophobic fraction could not be elu
ted by standard procedures. Only after severe hydrolysis and further e
xtraction was it possible to recover most of this material. These find
ings indicate that usual XAD-2 fractionation methods may result in lar
ge underestimates of the hydrophobic fraction of PDOC.