DETERMINATION OF ACRIDINE DERIVED COMPOUNDS IN CHARCOAL-GRILLED MEAT AND CREOSOTE OILS BY LIQUID-CHROMATOGRAPHIC AND GAS-CHROMATOGRAPHIC ANALYSIS

A method is described for the determination of basic nitrogen-containi ng polycyclic aromatic compounds (PANHs, azaarenes) in charcoal-grille d meat and creosote samples. The enrichment procedure includes liquid- liquid partition (cyclohexane), extraction of PANHs with acid and re-e xtraction with cyclohexane after neutralization. Further purification is performed by column chromatography on alumina. Basic fractions were analyzed by liquid chromatography (LC) with UV detection and capillar y gas chromatography (GC) with flame ionization detection (FID) and ma ss spectrometry (MS). The lowest detection limits ranged from 0.01 to 0.14 ng with GC-MS-SIM, allowing the determination of these compounds at the pg g(-1) level in meat samples.