Mpj. Szabo et Gh. Bechara, IMMUNIZATION OF DOGS AND GUINEA-PIGS AGAINST RHIPICEPHALUS-SANGUINEUSTICKS USING GUT EXTRACT, Veterinary parasitology, 68(3), 1997, pp. 283-294
Tick-bite naive guinea pigs were inoculated three times with Rhipiceph
alus sanguineus gut or salivary gland extracts and saponin as adjuvant
. Dogs were inoculated three times with gut extract only as this fract
ion induced a more efficient resistance in guinea pigs (lower tick rec
overy and lower engorged female weights). Freund's adjuvant and saponi
n were used as adjuvants for the immunisation of dogs. Freund's adjuva
nt was used to enhance cellular immunity. The highest level of resista
nce in dogs was induced by the immunisation with gut extract and Freun
d's adjuvant. Many female ticks from dogs immunised this way engorged
fully but died prior to oviposition. Resistant guinea pigs and dogs se
emed to trigger different immune mechanisms against R. sanguineus tick
s as damage to parasites also differed. A major role for cellular immu
nity in the resistance of dogs against R. sanguineus ticks is suggeste
d. Resistance mechanisms against R. sanguineus ticks is discussed.