INCREASE OF BOTH TRANSCRIPTION AND TRANSLATION ACTIVITIES FOLLOWING SEPARATE IRRADIATION OF THE IN-VITRO SYSTEM COMPONENTS WITH HE-NE-LASER

To gain insight into the mechanism by which cell irradiation with low power continuous wave He-Ne laser (632.8 nm) causes a general stimulat ion of biosynthetic properties, separate components of transcription a nd translation systems were irradiated (energy dose 2 Joules/cm(2); la ser power 12 mW) with measurements made of in vitro RNA and protein sy nthesis. Ln addition, all tested components were investigated with res pect to influence of laser irradiation on their conformation, as spect roscopically monitored. In all cases an increase in both transcription and translation activities was found with a significant change in abs orbance/fluorescence spectra. (C) 1994 Academic Press, Inc.