D. Nilsson et E. Johansen, A CONSERVED SEQUENCE IN TRANSFER-RNA AND RIBOSOMAL-RNA PROMOTERS OF LACTOCOCCUS-LACTIS, Biochimica et biophysica acta, N. Gene structure and expression, 1219(1), 1994, pp. 141-144
A tRNA operon (trnA) from Lactococcus lactis consisting of seven tRNA
genes and a 5S rRNA gene was cloned and sequenced. Promoter-fusion of
the trnA promoter to a promoter-less beta-galactosidase gene of Leucon
ostoc mesenteroides resulted in high levels of beta-galactosidase acti
vity in L. lactis. Searching for sequences with similarity to the sequ
ence of the promoter region revealed a consensus sequence of promoters
preceeding rRNA operons and tRNA operons from Lactococcus species inc
luding a not previously described conserved sequence (AGTT).