GLUTAMATE PRODUCTION BY CULTURED MICROGLIA - DIFFERENCES BETWEEN RAT AND MOUSE, ENHANCEMENT BY LIPOPOLYSACCHARIDE AND LACK EFFECT OF HIV COAT PROTEIN GP120 AND DEPOLARIZING AGENTS

Authors
Citation
M. Patrizio et G. Levi, GLUTAMATE PRODUCTION BY CULTURED MICROGLIA - DIFFERENCES BETWEEN RAT AND MOUSE, ENHANCEMENT BY LIPOPOLYSACCHARIDE AND LACK EFFECT OF HIV COAT PROTEIN GP120 AND DEPOLARIZING AGENTS, Neuroscience letters, 178(2), 1994, pp. 184-188
Citations number
21
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
03043940
Volume
178
Issue
2
Year of publication
1994
Pages
184 - 188
Database
ISI
SICI code
0304-3940(1994)178:2<184:GPBCM->2.0.ZU;2-O
Abstract
Glutamate release from rat and mouse microglia subcultures grown in a serum-free medium was substantially greater in the presence than in th e absence of a physiological concentration of glutamine (0.5 mM). Mous e microglia produced and released more glutamate than rat microglia. G lutamate accumulation in the medium increased with time and cell densi ty, which is consistent with the virtual absence of glutamate reuptake . Lipopolysaccharide (LPS; 10-100 ng/ml), HIV coat protein gp120 (0.1- 10 nM), high K+ (35 mM) or ATP (150 mu M), did not affect glutamate re lease from cells maintained in serum-free medium. In the presence of 1 % dialyzed serum, however, LPS induced a dose- and time-dependent incr ease in the accumulation of glutamate in the medium, suggesting that, as in other cell types, serum factors are required for LPS binding to its receptors.