RAPID AND SELECTIVE ENHANCEMENT OF DNA-BINDING ACTIVITY OF THE TRANSCRIPTION FACTOR AP1 BY SYSTEMIC ADMINISTRATION OF N-METHYL-D-ASPARTATE IN MURINE HIPPOCAMPUS
Y. Yoneda et K. Ogita, RAPID AND SELECTIVE ENHANCEMENT OF DNA-BINDING ACTIVITY OF THE TRANSCRIPTION FACTOR AP1 BY SYSTEMIC ADMINISTRATION OF N-METHYL-D-ASPARTATE IN MURINE HIPPOCAMPUS, Neurochemistry international, 25(3), 1994, pp. 263-271
Brain nuclear extracts of ddY mice contained 3 different transcription
factors with leucine-zipper domains, including activator protein-1 (A
P1), cyclic AMP responsive element binding protein (CREB) and Myc. An
intraperitoneal injection of N-methyl-D-aspartate (NMDA) was effective
in selectively inducing 6-fold enhancement of DNA binding activity of
AP1 in the hippocampus 2 h after the administration. Furthermore, NMD
A induced less than 2-fold potentiation of the AP1 binding in the stri
atum, hypothalamus, medulla-pons and cerebral cortex in a rank order o
f decreasing magnitude. However, the AP1 binding was not significantly
affected by the systemic injection of NMDA in the midbrain and cerebe
llum In contrast, NMDA virtually did not alter DNA binding activities
of both CREB and Myc in discrete structures of murine brain under simi
lar experimental conditions. These results suggest that the systemic a
dministration of NMDA may induce rapid and selective enhancement of DN
A binding activity of AP1 in murine hippocampus.