IMMUNOPHENOTYPING OF MURINE PERITONEAL-MACROPHAGES AND LYMPHOCYTES BYFLOW-CYTOMETRY

Authors
PLASMAN N VRAY B
Citation
N. Plasman et B. Vray, IMMUNOPHENOTYPING OF MURINE PERITONEAL-MACROPHAGES AND LYMPHOCYTES BYFLOW-CYTOMETRY, Journal of immunological methods, 174(1-2), 1994, pp. 123-131
Citations number
76
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
174
Issue
1-2
Year of publication
1994
Pages
123 - 131
Database
ISI
SICI code
0022-1759(1994)174:1-2<123:IOMPAL>2.0.ZU;2-#
Abstract
Peritoneal cells include mainly macrophages and both T and B lymphocyt es. We describe a simple procedure to analyze by flow cytometry the an tigens expressed on macrophage and/or lymphocyte membranes. F4/80 and CR3 macrophage antigens were detected with anti-F4/80 and anti-Mac-1 m onoclonal antibodies (mAb) respectively, CD4 and THY1.2 T lymphocyte a ntigens with GK1.5 and J1j mAbs respectively, B CD5 lymphocytes were i dentified with LY1.2 m4b and Fc gamma RII/Fc gamma RIII were detected with 2.4G2 mAb.