A. Wuyts et al., LEUKOCYTE RECRUITMENT BY MONOCYTE CHEMOTACTIC PROTEINS (MCPS) SECRETED BY HUMAN PHAGOCYTES, Journal of immunological methods, 174(1-2), 1994, pp. 237-247
Phagocyte recruitment is an important immunological phenomenon in infl
ammation arid cancer. A large family of selective chemotactic cytokine
s, designated chemokines, has recently emerged. Interleukin-8 (IL-8) i
s the prototype of such neutrophil activating factors, whereas MCP-1 i
s a well studied monocyte chemotactic protein. In vitro chemotactic as
says were used to isolate and identify natural chemokines from mononuc
lear phagocytes and tumor cells. Additional new chemotactic proteins (
MCP-2, MCP-3) attracting monocytes were also discovered by these metho
ds. All chemokines are structurally related and show affinity for hepa
rin. MCP-1, -2 and -3 have a comparable specific activity in monocyte
chemotaxis assays. Specific and sensitive radioimmunoassays for MCP-1
and IL-8 were developed to study the regulation of their secretion by
leukocytes. Monocytes or monocyte tumor cells produce MCP-1 and/or IL-
8 in response to cytokines, virus, double stranded RNA, bacterial endo
toxin, mitogen or phorbol ester. Granulocytes were found to secrete on
ly minor amounts of MCP-1 and IL-8.