LEUKOCYTE RECRUITMENT BY MONOCYTE CHEMOTACTIC PROTEINS (MCPS) SECRETED BY HUMAN PHAGOCYTES

Citation
A. Wuyts et al., LEUKOCYTE RECRUITMENT BY MONOCYTE CHEMOTACTIC PROTEINS (MCPS) SECRETED BY HUMAN PHAGOCYTES, Journal of immunological methods, 174(1-2), 1994, pp. 237-247
Citations number
59
Categorie Soggetti
Immunology
ISSN journal
00221759
Volume
174
Issue
1-2
Year of publication
1994
Pages
237 - 247
Database
ISI
SICI code
0022-1759(1994)174:1-2<237:LRBMCP>2.0.ZU;2-M
Abstract
Phagocyte recruitment is an important immunological phenomenon in infl ammation arid cancer. A large family of selective chemotactic cytokine s, designated chemokines, has recently emerged. Interleukin-8 (IL-8) i s the prototype of such neutrophil activating factors, whereas MCP-1 i s a well studied monocyte chemotactic protein. In vitro chemotactic as says were used to isolate and identify natural chemokines from mononuc lear phagocytes and tumor cells. Additional new chemotactic proteins ( MCP-2, MCP-3) attracting monocytes were also discovered by these metho ds. All chemokines are structurally related and show affinity for hepa rin. MCP-1, -2 and -3 have a comparable specific activity in monocyte chemotaxis assays. Specific and sensitive radioimmunoassays for MCP-1 and IL-8 were developed to study the regulation of their secretion by leukocytes. Monocytes or monocyte tumor cells produce MCP-1 and/or IL- 8 in response to cytokines, virus, double stranded RNA, bacterial endo toxin, mitogen or phorbol ester. Granulocytes were found to secrete on ly minor amounts of MCP-1 and IL-8.