M. Domeika et al., DIAGNOSIS OF GENITAL CHLAMYDIA-TRACHOMATIS INFECTIONS IN ASYMPTOMATICMALES BY TESTING URINE BY PCR, Journal of clinical microbiology, 32(10), 1994, pp. 2350-2352
An enzyme-linked immunosorbent assay (EIA) (MikroTrak; Syva) was compa
red with PCR (Amplicor; Roche) for detection of Chlamydia trachomatis
in first-void urine (FVU) Com 184 men attending a skin and venereal di
sease clinic. The prevalence of C. trachomatis in the population studi
ed was 18.5%. Discrepant results between Syva EIA and Roche PCR were r
etested by using major outer membrane protein primer-based PCR. After
retesting, the sensitivity, the specificity, and the positive and nega
tive predictive values for the Syva EIA were 85.3, 100, 100, and 77.5%
, respectively, and those for the Roche PCR 100, 100, 100, and 100%, r
espectively. It was concluded that PCR provides a highly sensitive and
specific noninvasive screening method for genital chlamydial infectio
n in asymptomatic men.