THE EFFECTIVENESS OF INACTIVATED, PURIFIED AND CONCENTRATED EXPERIMENTAL RABIES VACCINE FOR VETERINARY USE - IMMUNOGENIC ACTIVITY

The authors prepared an experimental, inactivated, concentrated and hi ghly purified rabies vaccine from the strain Vnukovo-32/107. The purif ication and concentration (simultaneous - in one operation) was carrie d out by affinity chromatography. The content of rabies antigen in the vaccine was 4.256 UE/cm(3) (determined by ELISA method). The authors used their own lipoid adjuvant - type oil in water - to resolve the ly ophilized vaccine to original volume. It is based on esters of fatty a cids. The effectiveness - immunogenic activity - of the experimental p reparation was compared with that of commercial rabies vaccines and th e reference vaccine by means of the NIH and NRLR methods. The results obtained (2.826 units/cm(3) by the NIH and 3.085 units/cm(3) by the NR LR method) confirmed that the method of affinity chromatography could be used to prepare a rabies vaccine, exhibiting effectiveness superior to commercial preparations available, and complying with the WHO crit eria.