THE ION-CHROMATOGRAPHIC BEHAVIOR OF ARSENITE, ARSENATE, METHYLARSONICACID AND DIMETHYLARSINIC ACID ON THE HAMILTON PRP-X100 ANION-EXCHANGECOLUMN

The HPLC separation of arsenite, arsenate, methylarsonic acid and dime thylarsinic acid has been studied in the past but not in a systematic manner. The dependence of the retention times of these arsenic compoun ds on the PH of the mobile phase, on the concentration and the chemica l composition of buffer solutions (phosphate, acetate, potassium hydro gen phthalate) and on the presence of sodium sulfate or nickel sulfate in the mobile phase was investigated using a Hamilton PRP-X100 anion- exchange column. With a flame atomic absorption detector and arsenic c oncentrations of at least 10 mg dm-3 all investigated mobile phases wi ll separate the four arsenic compounds at appropriate pH values in the range 4-8. The shortest analysis time (approximately 3 min) was achie ved with a 0.006 mol dm-3 potassium hydrogen phthalate mobile phase at pH 4, the longest (approximately 10 min) with 0.006 mol dm-3 sodium s ulfate at pH 5.9 at a flow rate of 1.5 cm3 min-1. With a graphite furn ace atomic absorption detector at the required, much lower, flow rate of approximately 0.2 cm3 min-1 acceptable separations were achievable only with the pH 6 phosphate buffer (0.03 mol dm-3) and the nickel sul fate solution (0.005 mol dm-3) as the mobile phase. To become detectab le approximately 100 ng arsenic from each arsenic compound (100 mul in jection) must be chromatographed with the phosphate buffer, and approx imately 10 ng with the nickel sulfate solution.