MAGNESIUM-IONS ARE REQUIRED FOR HEP-2 CELL-ADHESION BY ENTEROAGGREGATIVE STRAINS OF ESCHERICHIA-COLI O126-H27 AND O44-H18

Authors
CHART H SPENCER J SMITH HR ROWE B
Citation
H. Chart et al., MAGNESIUM-IONS ARE REQUIRED FOR HEP-2 CELL-ADHESION BY ENTEROAGGREGATIVE STRAINS OF ESCHERICHIA-COLI O126-H27 AND O44-H18, FEMS microbiology letters, 148(1), 1997, pp. 49-52
Citations number
13
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
148
Issue
1
Year of publication
1997
Pages
49 - 52
Database
ISI
SICI code
0378-1097(1997)148:1<49:MARFHC>2.0.ZU;2-R
Abstract
Enteroaggregative strains of Escherichia coli, belonging to serotypes O44:H18 and O126:H27, were used to show that magnesium ions were essen tial for the adhesion of these enteroaggregative strains to HEp-2 cell s. The removal of Mg2+ ions from culture media was correlated with the inability of strains to produce an outer membrane-associated protein of 18 kDa and a pellicle. It was concluded that magnesium ions were di rectly involved with the expression of an 18 kDa outer membrane associ ated protein by strains of E. coil O126:H27 and O44:H18, and that the outer membrane-associated protein was involved in both HEp-2 adhesion and pellicle formation.