ESTABLISHING A MODEL TO STUDY THE REGULATION OF THE LACTOSE OPERON INLACTOBACILLUS-CASEI

Authors
GOSALBES MJ MONEDERO V ALPERT CA PEREZMARTINEZ G
Citation
Mj. Gosalbes et al., ESTABLISHING A MODEL TO STUDY THE REGULATION OF THE LACTOSE OPERON INLACTOBACILLUS-CASEI, FEMS microbiology letters, 148(1), 1997, pp. 83-89
Citations number
24
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
148
Issue
1
Year of publication
1997
Pages
83 - 89
Database
ISI
SICI code
0378-1097(1997)148:1<83:EAMTST>2.0.ZU;2-4
Abstract
The chromosomally encoded lactose-specific phosphoenol pyruvate-depend ent phosphotransferase system (PTS) has been investigated in Lactobaci llus casei ATCC 393 [pLZ15(-)] and it was considered an excellent syst em to study the regulation of the lactose operon. This chromosomal ope ron has been cloned and sequenced, being 99% homologous to that encode d on the plasmid pLZ64. Expression of the lactose operon in different mutants of L. casei ATCC 393 [pLZ15(-)] and primer extension analysis revealed that it is subject to a dual regulation: (i) glucose repressi on possibly mediated by CcpA and PTS elements, and (ii) induction by l actose through transcriptional antitermination.