ON THE MOBILITY BEHAVIOR OF A CURVED DNA FRAGMENT LOCATED IN CIRCULARPERMUTATION

Experimental and theoretical investigations on the mobility behavior o f a set of permuted fragments with a K-DNA insert is reported. The fra gments with the permuted flanking sequences have the K-DNA insert loca ted differentially with respect to the fragment ends. The fragment whe rein the insert is located in the center showed maximum retardation as compared to fragments where the insert was at the end. The experiment al analysis is also in accord with the theoretical investigation.