Brucella ovis infection could not be eradicated from two flocks of she
ep despite serologically testing and culling the infected rams for fou
r years. The hypothesis that the ewes played a major role in the maint
enance of the infection in both flocks was investigated by using serol
ogical, bacteriological and pathological criteria. Specific antibodies
against B ovis were demonstrated in 71 ewes in the two flocks. Forty-
four of the seropositive ewes were slaughtered for bacteriological and
pathological studies and B ovis was isolated from 16 of them; the ute
rus was the target organ of the infection, although extrauterine infec
tion was also demonstrated in some of the infected ewes.