Three new alkaloids, namely, 4,6-dien-3-oxo-N-demethylconanine (pubesc
ine), 1,4-dien-3-oxo-N-demethylconanine (norholadiene) and 3-dimethyla
mino-5-en-12 alpha-hydroxyconanin (pubescimine), have been isolated fr
om the bark of Holarrhena pubescens and their structures elucidated th
rough exhaustive 1D and 2D NMR (COSY-45, NOESY, J-resolved and HMQC).