The ethylene evolution of germinating peas Pisum savitum L. exposed to
elevated ozone levels is studied using a CO2 laser-driven photoacoust
ic detection system. For ozone fumigation experiments a specific flow
system is developed resulting in adjustable, controlled ozone concentr
ations between 270 and 10 ppbv in air. Ozone fumigation enhances ethyl
ene production, even before the onset of visible germination, and inhi
bits growth as compared to non-fumigated pea seedlings. The combinatio
n of ozone fumigation and application of the ACC-synthase inhibitor AV
G still yields increased ethylene levels indicating an alternative pat
hway for enhanced ethylene production.