RESTRICTED VARIABLE REGION GENE USAGE AND POSSIBLE RHEUMATOID-FACTOR RELATIONSHIP AMONG HUMAN MONOCLONAL-ANTIBODIES SPECIFIC FOR THE AD-1 EPITOPE ON CYTOMEGALOVIRUS GLYCOPROTEIN-B

Citation
M. Ohlin et al., RESTRICTED VARIABLE REGION GENE USAGE AND POSSIBLE RHEUMATOID-FACTOR RELATIONSHIP AMONG HUMAN MONOCLONAL-ANTIBODIES SPECIFIC FOR THE AD-1 EPITOPE ON CYTOMEGALOVIRUS GLYCOPROTEIN-B, Molecular immunology, 31(13), 1994, pp. 983-991
Citations number
33
Categorie Soggetti
Immunology,Biology
Journal title
ISSN journal
01615890
Volume
31
Issue
13
Year of publication
1994
Pages
983 - 991
Database
ISI
SICI code
0161-5890(1994)31:13<983:RVRGUA>2.0.ZU;2-V
Abstract
The nucleotide sequences of the variable region genes encoding five di fferent human, high affinity antibodies, specific for the major neutra lization determinant (AD-1) expressed by human cytomegalovirus glycopr otein B (gp58/116), have been determined. Three of the five heavy chai n variable regions belonged to the small VHV-family, although they com bined with a diverse set of light chains (V(kappa)IIIb, VlambdaII and VlambdaIII). The other two antibodies belonged to V-H-families III and IV. One of the VHV-family genes most likely originated from a previou sly unreported germline gene or allele, since it carries a nine nucleo tide insert in framework 1. In addition, V-lambda-genes showed variabl e homology (77-95%) to known germline sequences, while V-kappa-genes s howed high homology (approximately 98%) with their proposed germline o rigin. Despite the close homology of the V(kappa)IIIb-gene used to exp ress one of the antibodies with its corresponding germline gene, the p rotein did not strongly express some idiotypes associated with this li ght chain family. There is, thus, no direct relation between the expre ssion of these crossreactive idiotypes and the use of even modestly mu tated light chains belonging to this V-kappa-family, which has been im plicated in the development of anti-idiotypic networks possibly induci ng autoantibodies, such as rheumatoid factors.