THE SEQUENCE OF 12-CENTER-DOT-5 KB FROM THE RIGHT ARM OF CHROMOSOME-II PREDICTS A NEW N-TERMINAL SEQUENCE FOR THE IRA1 PROTEIN AND REVEALS 2 NEW GENES, ONE OF WHICH IS A DEAD-BOX HELICASE

We have determined the complete nucleotide sequence of a 12.5 kb segme nt from the right arm of chromosome II carried by the cosmid alpha 20. The sequence encodes the 5' end of the IRA1 gene. Two complete new op en reading frames and the 3' non-coding region of the SUP1 (SUP45) gen e. A comparison of our sequence with the data bank reveals a 154 amino acid extension at the N-terminus of Ira1p compared to the previously predicted sequence. According to the 11th edition of the Saccharomyces cerevisine genetic map, our sequence should encode the MAK5 gene, whi ch is necessary for the maintenance of dsRNA killer plasmids. One of t he two new open reading frames, YBR1119, is predicted to encode an RNA helicase, thus YBR1119 may correspond to the MAKS gene. The sequence has been deposited in the EMBL data library under Accession Number X78 937.