DETERMINATION OF THE SUBSTITUENT PATTERN OF HETEROGENEOUSLY AND HOMOGENEOUSLY SYNTHESIZED CARBOXYMETHYL CELLULOSE BY USING HIGH-PERFORMANCELIQUID-CHROMATOGRAPHY
T. Heinze et al., DETERMINATION OF THE SUBSTITUENT PATTERN OF HETEROGENEOUSLY AND HOMOGENEOUSLY SYNTHESIZED CARBOXYMETHYL CELLULOSE BY USING HIGH-PERFORMANCELIQUID-CHROMATOGRAPHY, Die Angewandte makromolekulare Chemie, 215, 1994, pp. 93-106
A rapid and convenient procedure was developed for the determination o
f the substituent pattern of carboxymethyl cellulose (CMC) by means of
high-performance liquid chromatography. The hydrolysates of CMC are s
eparated on a polystyrene-based strong cation-exchange resin into gluc
ose, 2,3,6-tri-O-carboxymethyl glucose and groups of 2-, 3- and 6-mono
-O- and 2,3-, 2,6-, and 3,6-di-O-carboxymethyl glucoses. By this metho
d the determination of the average degree of substitution as well as t
he substituent pattern is possible. In this way we found that CMC samp
les which were synthesized in LiCl/N,N-dimethylacetamide as a cellulos
e solvent, contain a significantly higher amount of both tricarboxymet
hylated and unsubstituted units than those obtained in a slurry of cel
lulose in isopropanol/water at comparable DS values. The substituent p
attern of heterogeneously synthesized CMC is mainly determined by stat
istics.