QUANTIFICATION OF HISTAMINE BY POSTCOLUMN FLUORESCENCE DETECTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ORTHOPHTHALALDEHYDE IN TETRAHYDROFURAN AND REACTION-MECHANISM

Authors
KURUMA K HIRAI E UCHIDA K KIKUCHI J TERUI Y
Citation
K. Kuruma et al., QUANTIFICATION OF HISTAMINE BY POSTCOLUMN FLUORESCENCE DETECTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ORTHOPHTHALALDEHYDE IN TETRAHYDROFURAN AND REACTION-MECHANISM, Analytical sciences, 10(2), 1994, pp. 259-265
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical sciences → ACNP
ISSN journal
09106340
Volume
10
Issue
2
Year of publication
1994
Pages
259 - 265
Database
ISI
SICI code
0910-6340(1994)10:2<259:QOHBPF>2.0.ZU;2-2
Abstract
For quantification of histamine, we developed a highly specific and se nsitive method of postcolumn fluorescence detection HPLC using a tetra hydrofuran solution of orthophthalaldehyde. Its detection limit was 2 - 5 pg. This method was applied to the measurement of histamine in hum an blood and urine samples. Analysis by LC/MS and NMR suggested that t he fluorophore in this method has an isoindole structure.